Luminescent assays


Aboatox Biotox Kit for Luminescent Toxicity Screening

Aqualytic provides Water Testing Equipment Pretoria based. Our BioTox™ Kit provides freeze-dried reagents for determination of the inhibitory effect of water samples, solid samples and detergent residuals on the light emission of Vibrio fischeri NRRL-B 11177.
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Test Principle

The test bacteria produce light as part of their natural metabolism. Toxic compounds interfere with these metabolic processes resulting in a reduction of the light emission. The changes in light output are measured with luminometer and the decrease of the luminescence after a contact of less than 30 minutes equate to the acute toxicity level of the sample.


Biothema Portable Hygiene Laboratory

This Water Testing Equipment kit is intended for determination of ATP (adenosine triphosphate) on surfaces or in liquids. All living cells contain ATP. When cells die of natural causes, ATP is normally degraded. Cleaning and disinfecting a surface, which has been in contact with biological matter, may leave small amounts of residues containing ATP. Such residues will provide an excellent growth medium for airborne or other micro-organisms. Even if the surface is sterile after cleaning and disinfecting, it may contain large numbers of micro-organisms within a few hours. Determination of ATP on a surface will tell how much biological contamination was left after cleaning and disinfecting. The kit can also be used for determination of ATP in liquids.

 

Assay principle

Before the assay, ATP must be released from the biological material. This is achieved with Extractant B/S, which extracts ATP from most cell types of animal as well as bacterial origin. Extractant B/S is applied onto the surface with a dropper bottle and is evenly distributed over the surface with a swab. The active ingredients penetrate all materials on the surface including biofilms. The swab will absorb ATP from the surface.

The ATP content of the swab is determined by rotating the swab in a microtube containing an ATP reagent. This reagent contains luciferase and luciferin resulting in the emission of light. The intensity of the light is proportional to the amount of ATP and is measured in a luminometer. After adding a known amount of ATP standard the light is measured again. This makes it possible to calculate the amount of ATP in unknown samples and to express the result in moles (the chemical unit for mass).

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